mRNA碱基修饰检测

  • 简介
  • 分析种类
  • 结果展示

  • mRNA化学修饰的定量检测是表观转录组学研究所必不可少的环节。检测不同处理条件下的表观转录修饰变化将为下一步的研究提供重要线索。Aksomics(原康成生物)的质谱检测平台能够为客户提供全面而准确的mRNA修饰谱。利用Agilent质谱仪,检测35种修饰碱基在各个样品中的修饰丰度。



    服务特色:

    ● 优化的前期处理与Agilent优质设备 

    ● 全面而准确的碱基修饰分析


    分析流程:

    LC-MS mRNA修饰检测服务流程图

    图释:LC-MS mRNA修饰检测服务流程图。


  • Number Nucleoside Symbol Number Nucleoside Symbol
    01 3′-O-methyladenosine 3′-OMeA 27 3'-O-methyluridine 3'-OMeU
    02 2′-O-methylcytidine Cm 28 5-methyl-2-thiouridine m5s2U
    03 3-methylcytidine m3C 29 5-methoxyuridine mo5U
    04 5-methylcytidine m5C 30 pseudouridine Ψ
    05 N6-isopentenyladenosine i6A 31 2'-O-methylinosine Im
    06 5,2'-O-dimethylcytidine m5Cm 32 3-methyluridine m3U
    07 1-methyladenosine m1A 33 1-methylpseudouridine m1Ψ
    08 2-thiocytidine s2C 34 5-hydroxymethylcytidine hm5C
    09 N2,N2,7-trimethylguanosine m2,2,7G 35 5,2'-O-dimethyluridine m5Um
    10 N4-acetyl-2'-O-methylcytidine ac4Cm 36 N6-threonylcarbamoyladenosine t6a
    11 N6-methyladenosine m6A 37 2-methylthio-N6-threonylcarbamoyladenosine ms2t6A
    12 3'-O-methylcytidine 3′-OMeC 38 5-carboxymethyluridine cm5U
    13 2'-O-methyladenosine Am 39 5-methoxycarbonylmethyl-2-thiouridine mcm5s2U
    14 N2, N2-dimethylguanosine m22G 40 5-Methoxycarbonylmethyluridine mcm5U
    15 5'-O-methylthymidine 5′-OMeT 41 2-methylthio-N6-isopentenyladenosine ms2i6A
    16 2′-O-methyluridine Um 42 Peroxywybutosine o2Yw
    17 inosine I 43 5-taurinomethyl-2-thiouridine tm5s2U
    18 2′-O-methylguanosine Gm 44 5-oxyacetic acid uridine cmo5U
    19 1-methylguanosine m1G 45 5-carbamoylmethyuridine ncm5U
    20 7-methylguanosine m7G 46 Queuosine Q
    21 N2-methylguanosin m2G 47 5-taurinomethyluridine tm5U
    22 3'-O-methylinosine 3'-OMeI 48 5-formyl-2′-O-methylcytidine f5Cm
    23 2-thiouridine s2U 49 dihydrouridine D
    24 4-thiouridine s4U 50 5-formylcytidine f5c
    25 5-methyluridine m5U 51 wybutosine yW
    26 N4-acetylcytidine ac4C 52 5-methoxycarbonylmethyl-2'-o-methyluridine mcm5Um
  • 1. 修饰定量分析

    mRNA修饰数量分析图

    图释:mRNA修饰数量分析图。


    2. 总离子流程图

    总离子流程图

    图释:总离子流程图